bs-1722R [Primary Antibody]
E2F3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E2F3

Immunogen Range: 381-465/465


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1871

Swiss Prot: O00716

Source: KLH conjugated synthetic peptide derived from human E2F3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 49


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang et al. miR-203a suppresses cell proliferation by targeting E2F transcription factor 3 in human gastric cancer. (2017) Oncol.Lett. 14:7687-7690Read more>>
  • Yuan et al. Methylation by NSun2 represses the levels and function of microRNA 125b. (2014) Mol.Cell.Biol. 34:3630-41Read more>>
  • Aditi Karmakar. et al. Identification of Epigenetically Modified Hub Genes and Altered Pathways Associated With Retinoblastoma. Front Cell Dev Biol. 2022; 10: 743224Read more>>
VALIDATION IMAGES

Mouse Cerebrum lysates probed with E2F3 Polyclonal Antibody, Unconjugated (bs-1722R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.