bs-1727R [Primary Antibody]
ABCG4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ABCG4

Immunogen Range: 551-646/646


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 64137

Swiss Prot: Q9H172

Source: KLH conjugated synthetic peptide derived from human ABCG4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May be involved in macrophage lipid homeostasis.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Matsumoto, Koichi, et al. "Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood–cerebrospinal fluid barrier in human brain." Histochemistry and Cell Biology (2015): 1-15.Read more>>
  • Ueno, Masaki, et al. "Blood–brain barrier and blood–cerebrospinal fluid barrier in normal and pathological conditions." Brain Tumor Pathology (2016): 1-8.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-ABCG4, Unconjugated(bs-1727R) followed by conjugation to the secondary antibody and DAPI staining\n


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ABCG4 Polyclonal Antibody, Unconjugated (bs-1727R) at 1:400 overnight at 4°C, DAB staining.