bs-1734R [Primary Antibody]
E2F5 Polyclonal Antibody
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Host: Rabbit

Target Protein: E2F5

Immunogen Range: 101-200/346


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1875

Swiss Prot: Q15329

Source: KLH conjugated synthetic peptide derived from human E2F5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Jin, et al. "Analysis of genetic aberrations on chromosomal region 8q21?C24 identifies E2F5 as an oncogene with copy number gain in prostate cancer."Medical Oncology 30.1 (2013): 1-10..Read more>>
  • Lina Li. et al. E2F5 promotes proliferation and invasion of gastric cancer through directly upregulating UBE2T transcription. Digest Liver Dis. 2021 SepRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti E2F5 Polyclonal Antibody, Unconjugated (bs-1734R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


RSC96 cells probed with E2F5 Polyclonal Antibody, Unconjugated (bs-1734R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).