bs-17376R [Primary Antibody]
CBX4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CBX4

Immunogen Range: 401-500/560


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8535

Swiss Prot: O00257

Source: KLH conjugated synthetic peptide derived from human CBX4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Involved in the sumoylation of HNRNPK, a p53/TP53 transcriptional coactivator, hence indirectly regulates p53/TP53 transcriptional activation resulting in p21/CDKN1A expression. Monosumoylates ZNF131. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Han, Xiao, et al. "HIF-1α SUMOylation affects the stability and transcriptional activity of HIF-1α in human lens epithelial cells." Graefe's Archive for Clinical and Experimental Ophthalmology 253.8 (2015): 1279-1290.Read more>>
  • Xu J et al. Regulatory effect of two Trichinella spiralis serine protease inhibitors on the host's immune system. Sci Rep. 2019 Nov 19;9(1):17045.Read more>>
  • Hu C et al. CBX4 promotes the proliferation and metastasis via regulating BMI‐1 in lung cancer. J Cell Mol Med. 2019 Nov 13. Read more>>
VALIDATION IMAGES

Lane 1: Mouse testis lysates; Lane 2: Human HepG2 lysates probed with CBX4 Polyclonal Antibody, Unconjugated (bs-17376R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Human HL-60 cell lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human HUVEC cell lysates; Lane 8: Human A549 cell lysates probed with CBX4 Polyclonal Antibody, Unconjugated (bs-17376R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (bs-17376R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.