bs-1739R [Primary Antibody]
Wnt1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Wnt1

Immunogen Range: 251-350/370


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7471

Swiss Prot: P04628

Source: KLH conjugated synthetic peptide derived from human Wnt1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Hong, et al. "Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprungs disease." International Journal of Experimental Pathology (2014).Read more>>
  • Shan, Shumei, et al. "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." Int J Clin Exp Pathol 8.10 (2015): 12357-12367.Read more>>
  • Sung, Hsin-Ju Chiang, et al. "Combined therapy with melatonin and exendin-4 effectively attenuated the deterioration of renal function in rat cardiorenal syndrome." American Journal of Translational Research 9.2 (2017): 214-229.Read more>>
  • Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular medicine reports 12.2 (2015): 1971-1976.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat brain labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse brain lysates probed with Rabbit Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.