Host: Rabbit
Target Protein: ECE2
Immunogen Range: 601-700/883
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 110599583; 9718
Swiss Prot: P0DPD8
Source: KLH conjugated synthetic peptide derived from human ECE2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 80-100
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Esposito, Giuseppe, et al. "Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration." Scientific reports 6 (2016).Read more>>
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ECE2 Polyclonal Antibody, Unconjugated (bs-1753R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: 293T cell lysates probed with ECE2 Polyclonal Antibody, Unconjugated (bs-1753R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.