Host: Rabbit
Target Protein: Elastin
Immunogen Range: 681-786/786
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2006
Swiss Prot: P15502
Source: KLH conjugated synthetic peptide derived from human Elastin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Konii, Hanae, et al. "Stimulatory Effects of Cardiotrophin 1 on Atherosclerosis."Hypertension?(2013): HYPERTENSIONAHA-113Read more>>
- Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).Read more>>
- Hasegawa et al. Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo. (2014) PLoS.On. 9:e110866Read more>>
- Tong Xu. et al. Lithium chloride represses abdominal aortic aneurysm via regulating GSK3/SIRT1/NF-B signaling pathway. Free Radical Bio Med. 2021 Apr;166:1Read more>>
- Akira Minami. et al. Enhancement of elastin expression by transdermal administration of sialidase isozyme Neu2. Sci Rep-Uk. 2021 Feb;11(1):1-9Read more>>
- Hao Chen. et al. MicroRNA-124-3p affects myogenic differentiation of adipose-derived stem cells by targeting Caveolin-1 during pelvic floor dysfunction in Sprague Dawley rats. Ann Transl Med. 2021 Jan; 9(2): 161Read more>>
- Mi Hee Kwack. et al. Efficacy of a home-used high-intensity focused ultrasound device on wrinkle reduction. SKIN RES TECHNOL. 2022 Dec;29(1):e13266Read more>>
Human A549 cells probed with Rabbit Anti-Elastin Polyclonal Antibody (bs-1756R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated (bs-1756R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (bs-1756R) at 1:1000 4 °C temperature overnight 4 °C followed by conjugation to secondary antibody.
Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size: 66kD
Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated(bs-1756R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Uterus lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Rat Lung cell lysates; Lane 6: Rat Liver lysates; Lane 7: Rat Testis lysates; Lane 8: Human SH-SY5Y cell lysates; Lane 9: Human A549 cell lysates probed with Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Elastin) polyclonal Antibody, Unconjugated (bs-1756R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.