VALIDATION IMAGES
Mouse lung lysates probed with Elastin Polyclonal Antibody, unconjugated (bs-1756R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (bs-1756R) at 1:1000 4 °C temperature overnight 4 °C followed by conjugation to secondary antibody.
Formalin-fixed and paraffin embedded human artery labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human glioma labeled with Anti-Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse intestine lysates L2 rat lung lysates probed with Anti Alpha Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 66kD. Observed band size: 66kD
Formalin-fixed and paraffin embedded rat intervertebral disk labeled with Anti-ELASTIN, Unconjugated(bs-1756R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Uterus lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse NIH/3T3 cell lysates; Lane 5: Rat Lung cell lysates; Lane 6: Rat Liver lysates; Lane 7: Rat Testis lysates; Lane 8: Human SH-SY5Y cell lysates; Lane 9: Human A549 cell lysates probed with Elastin Polyclonal Antibody, Unconjugated (bs-1756R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.