Host: Rabbit
Target Protein: p21-ARC
Immunogen Range: 2-100/178
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 10094
Swiss Prot: O15145
Source: KLH conjugated synthetic peptide derived from human p21-ARC
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 20
Cross Reactive Species:
Human
Mouse
Xenopus laevis
Predicted Cross Reactive Species:
Rat
Cow
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
Line1,mouse intestinal lysate; Line2, Raji lysate probed with Rabbit Anti-p21-ARC Polyclonal Antibody, Unconjugated (bs-17589R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Lane 1: Spleen lysates probed with p21-ARC Polyclonal Antibody, Unconjugated (bs-17589R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with p21-ARC Polyclonal Antibody, Unconjugated (bs-17589R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with p21-ARC Polyclonal Antibody, Unconjugated (bs-17589R) at 1:200 overnight at 4°C, DAB staining.