bs-17591R-PE [Conjugated Primary Antibody]
phospho-JNK1 (Thr183) Polyclonal Antibody, PE Conjugated
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Host: Rabbit

Target Protein: phospho-JNK1 (Thr183)

Immunogen Range: 170-190/427


Modification Site: Thr183

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5599

Swiss Prot: P45983

Source: KLH conjugated synthesised phosphopeptide derived from human JNK1 around the phosphorylation site of Thr183

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

Conjugation: PE

Excitation/ Emission: 496,564nm/578nm

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)

Predicted Molecular Weight: 50 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chang L et al. Melamine causes testicular toxicity by destroying blood-testis barrier in piglets.Toxicol Lett. 2018 Oct 15;296:114-124. Read more>>
  • Wang X et al. Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells. Reprod Fertil Dev. 2020 Feb 6. Read more>>
  • Jian Wang. et al. Lactobacillus salivarius ameliorated Mycoplasma gallisepticum-induced inflammatory injury and secondary Escherichia coli infection in chickens: Involvement of intestinal microbiota. Vet Immunol Immunop. 2021 Mar;233:110192Read more>>
  • Jian Wang. et al. A respiratory commensal bacterium acts as a risk factor for Mycoplasma gallisepticum infection in chickens. Vet Immunol Immunop. 2020 Dec;230:110127Read more>>
  • Jing Li. et al. Melatonin ameliorates cypermethrin-induced impairments by regulating oxidative stress, DNA damage and apoptosis in porcine Sertoli cells. Theriogenology. 2021 MaRead more>>
  • Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;()Read more>>
  • Yusong Miao. et al. Methylsulfonylmethane ameliorates inflammation via NF-_B and ERK/JNK-MAPK signaling pathway in chicken trachea and HD11 cells during Mycoplasma gallisepticum infection. Poultry Sci. 2022 Jan;:101706Read more>>
  • Liu Jiawei. et al. Lactobacillus rhamnosus GR-1 Alleviates Escherichia coli-Induced Inflammation via NF-_B and MAPKs Signaling in Bovine Endometrial Epithelial Cells. Front Cell Infect Mi. 2022 Mar;0:199Read more>>
  • Meiqiong Wu. et al. Suppression of NADPH oxidase 4 inhibits PM2.5-induced cardiac fibrosis through ROS-P38 MAPK pathway. SCI TOTAL ENVIRON. 2022 Apr;:155558Read more>>
  • Zhang, Di. et al. Salvia miltiorrhiza polysaccharides ameliorates Staphylococcus aureus-induced mastitis in rats by inhibiting activation of the NF-_B and MAPK signaling pathways. BMC VET RES. 2022 Dec;18(1):1-11Read more>>
  • Shuting Wei. et al. Particle matters induce airway epithelial barrier dysfunction in vivo and in vitro: from a more realistic inhalation scenario. ENVIRON SCI-NANO. 2022 JuRead more>>
  • M. Zhang. et al. Osteocalcin reduces fat accumulation and inflammatory reaction by inhibiting ROS-JNK signal pathway in chicken embryonic hepatocytes. POULTRY SCIENCE. 2022 Jun;:102026Read more>>
  • Gao, Pei. et al. S1PR1 regulates NDV-induced IL-1_ expression via NLRP3/caspase-1 inflammasome. VET RES. 2022 Dec;53(1):1-13Read more>>
  • Dan-Ping Xie. et al. Peroxiredoxin 5 protects HepG2 cells from ethyl -carboline-3-carboxylate-induced cell death via ROS-dependent MAPK signalling pathways. J CANCER. 2022 Sep 6;13(11):3258-3267Read more>>
  • Cong Changsheng. et al. Renin-angiotensin system inhibitors mitigate radiation pneumonitis by activating ACE2-angiotensin-(1C7) axis via NF-B/MAPK pathway. SCI REP-UK. 2023 May;13(1):1-11Read more>>
  • Cai Juncheng. et al. NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway. VET RES. 2023 Dec;54(1):1-15Read more>>
  • Pei Luo. et al. Heat enhances the inhibitory effect of lipopolysaccharide on duck granulosa cell proliferation and steroid biosynthesis in vitro. ANIM SCI J. 2023 Jul;94(1):e13851Read more>>
VALIDATION IMAGES