DATASHEET
Host:
Rabbit
Target Protein:
MPND
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
Q3TV65
Swiss Prot:
68047
Source:
KLH conjugated synthetic peptide derived from human MPND
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Proteases are enzymes that are involved in protein catabolism by hydrolyzing the peptide bonds between amino acids in a polypeptide chain. MPND (MPN domain-containing protein) is a 471 amino acid protein that is thought to be a protease. Expressed as two isoforms produced by alternative splicing, MPND contains a JAMM motif and one MPN domain. The gene that encodes MPND maps to human chromosome 19, which consists of around 63 million bases with over 1,400 genes, making up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fcα receptors. Key genes for eye color and hair color also map to chromosome 19.
VALIDATION IMAGES
Lane 1: SH-SY5Y cell lysates probed with MPND Polyclonal Antibody, Unconjugated (bs-17737R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with MPND polyclonal antibody, unconjugated (bs-17737R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.