bs-1789R [Primary Antibody]
IL-12 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-12

Immunogen Range: 51-150/219


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3592

Swiss Prot: P29459

Source: KLH conjugated synthetic peptide derived from human IL-12

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)

Predicted Molecular Weight: 23


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Kang, et al. "CD14 overexpression upregulates TNF-??-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells."Molecular and cellular biochemistry 376.1-2 (2013): 137-143.3Read more>>
  • Friedemann, Markus, et al. "Instructing Human Macrophage Polarization by Stiffness and Glycosaminoglycan Functionalization in 3D Collagen Networks." Advanced Healthcare Materials (2017).Read more>>
  • Foulon M et al. Mycolactone toxin induces an inflammatory response by targeting the IL-1beta pathway: Mechanistic insight into Buruli ulcer pathophysiologyPLoS Pathog.2020 Dec 18;16(12):e1009107.Read more>>
VALIDATION IMAGES

paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-IL12 Polyclonal Antibody, Unconjugated (bs-1789R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAPI staining


U251 lysates probed with IL12 Polyclonal Antibody, Unconjugated (bs-1789R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.