bs-1808R [Primary Antibody]
Hpt/Haptoglobin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Hpt/Haptoglobin

Specificity: This protein may interact with Peptidase S1 Domain in LPA.

Immunogen Range: 251-350/406


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3240

Swiss Prot: P00738

Source: KLH conjugated synthetic peptide derived from human Hpt

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 27/43


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kälsch, Julia, et al. "Bathing in carbon dioxide-enriched water alters protein expression in keratinocytes of skin tissue in rats." International Journal of Biometeorology (2016): 1-8.Read more>>
  • Changsheng Xing. et al. Microbiota regulate innate immune signaling and protective immunity against cancer. Cell Host Microbe. 2021 ApRead more>>
VALIDATION IMAGES

L1 rat lung lysates L2 rat liver lysates probed with Anti Hpt/Haptoglobin Polyclonal Antibody, Unconjugated (bs-1808R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 44kD. Observed band size: 38kD,44kD


Lane 1: Hela cell lysates probed with Hpt/Haptoglobin Polyclonal Antibody, Unconjugated (bs-1808R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.