bs-1829R [Primary Antibody]
IL-15 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-15

Immunogen Range: 81-162/162


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3600

Swiss Prot: P40933

Source: KLH conjugated synthetic peptide derived from human IL-15

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 12.7


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yaiza M. Arenas. et al. Golexanolone improves fatigue, motor incoordination and gait and memory in rats with bile duct ligation. LIVER INT. 2023 NovRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colitis tissue labeled with IL-15 Polyclonal Antibody, Unconjugated (bs-1829R) at 1:200 dilution, followed by conjugation to the secondary antibody and DAB staining.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IL-15) polyclonal Antibody, Unconjugated (bs-1829R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.