bs-1833R [Primary Antibody]
VAT Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VAT

Immunogen Range: 201-300/532


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10493

Swiss Prot: Q99536

Source: KLH conjugated synthetic peptide derived from human VAT

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 57


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shen, Zhou. et al. Bone marrow mesenchymal stem cells therapy on bilateral pelvic nerve crush-induced voiding dysfunction in rats. INT UROGYNECOL J. 2022 Apr;:1-8Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-VAT Polyclonal Antibody, Unconjugated (bs-1833R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: rat brain lysates probed with Rabbit Anti-VAT Polyclonal Antibody, Unconjugated (bs-1833R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human SH-SY5Y cell lysates probed with Vesicular Acetylcholine Transporter Polyclonal Antibody, Unconjugated (bs-1833R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.