bs-1848R [Primary Antibody]
alpha MSH Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: alpha MSH

Immunogen Range: 138-150/267


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5443

Swiss Prot: P01189

Source: KLH conjugated synthetic peptide of human Alpha-MSH

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 1.4


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jussila, Anna, et al. "Narrow-band ultraviolet B radiation induces the expression of β-endorphin in human skin in vivo." Journal of Photochemistry and Photobiology B: Biology (2016).Read more>>
  • Andoh T et al. Involvement of α-Melanocyte-Stimulating Hormone-Thromboxane A2 System on Itching in Atopic Dermatitis. Am J Pathol. 2019 Jun 17. pii: S0002-9440(18)31174-X.Read more>>
  • Yurie Shimoda-Komatsu. et al. Digital immunohistological dissection of immune privilege collapse in syringotropic autoimmune diseases: Implication for the pathogenesis. J Dermatol Sci. 2021 Jan;101:3Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse foot tumor labeled with Anti-α-MSH, Unconjugated (bs-1848R) followed by conjugation to the secondary antibody


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Annexin A13 Polyclonal Antibody, Unconjugated (bs-12478R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse lymphoma labeled with Rabbit Anti alpha MSH Polyclonal Antibody, Unconjugated (bs-1848R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha MSH Polyclonal Antibody, Unconjugated (bs-1848R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.


Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha MSH Polyclonal Antibody, Unconjugated (bs-1848R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.