bs-1854R [Primary Antibody]
MMP-12 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MMP-12

Immunogen Range: 201-300/477


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 117033

Swiss Prot: Q63341

Source: KLH conjugated synthetic peptide derived from rat MMP12

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3 (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 42/52


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Papakonstantinou et al. Acute exacerbations of COPD are associated with significant activation of matrix metalloproteinase 9 irrespectively of airway obstruction, emphysema and infection. (2015) Respir.Res. 16:78Read more>>
  • Hashimoto K et al. Dietary DNA Attenuates the Degradation of Elastin Fibers in the Aortic Wall in Nicotine-Administrated Mice. (2018) Journal of Nutritional Science and Vitaminology, 64(4), 271–276. Read more>>
  • Sukketsiri W et al. Isoflavone Ameliorated Oxidative Stress and Vascular Damages in Nicotine-Administrated Mice. J Oleo Sci. 2019 Dec 1;68(12):1241-1249.Read more>>
  • ai S et al. Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD. Sci Rep. 2020 Jan 15;10(1):420. Read more>>
  • Mingzhu Xiao. et al. Nuciferine attenuates atherosclerosis by regulating the proliferation and migration of VSMCs through the Calm4/MMP12/AKT pathway in ApoE(-/-) mice fed with High-Fat-Diet. PHYTOMEDICINE. 2023 Jan;108:154536Read more>>
  • Hirona Kugo. et al. Tricaprin can prevent the development of AAA by attenuating aortic degeneration. BIOMED PHARMACOTHER. 2023 Apr;160:114299Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse ovarian tissue labeled with Anti-MMP-12, Unconjugated (bs-1854R) followed by conjugation to the secondary antibody


Human Raji lysates probed with MMP12 Polyclonal Antibody, Unconjugated (bs-1854R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.