Host: Rabbit
Target Protein: Ntn1/Netrin 1
Immunogen Range: 501-604/604
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9423
Swiss Prot: O95631
Source: KLH conjugated synthetic peptide derived from human NtN1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 64
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Pig
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Ko CL et al. Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. High Alt Med Biol. 2019 Jul 22. Read more>>
- Chhabra V et al. Hypobaric hypoxia induced renal damage is mediated by altering redox pathway.PLoS One. 2018 Jul 13;13(7):e0195701. Read more>>
- Zongding Wang. et al. Netrin-1 promotes liver regeneration possibly by facilitating vagal nerve repair after partial hepatectomy in mice. Cell Signal. 2022 Mar;91:110227Read more>>
- Victor H. Guaiquil. et al. Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury. EXP EYE RES. Exp Eye Res. 2022 Apr;:109054Read more>>
Mouse heart lysates probed with Rabbit Anti-Netrin 1 Polyclonal Antibody, Unconjugated (bs-1858R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse hippocampus labeled with Anti- Ntn1, Unconjugated (bs-1858R) followed by conjugation to the secondary antibody
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Netrin 1 Polyclonal Antibody, Unconjugated (bs-1858R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Netrin 1 Polyclonal Antibody, Unconjugated (bs-1858R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
SHSY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Netrin 1) polyclonal Antibody, Unconjugated (bs-1858R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.