bs-1867R [Primary Antibody]
PD-1/CD279 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PD-1/CD279

Immunogen Range: 201-288/288

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5133

Swiss Prot: Q15116

Source: KLH conjugated synthetic peptide derived from human PD-1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 32

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Song-An, et al. "Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway." Tumor Biology 39.3 (2017): 1010428317692237.Read more>>
  • Szabó, Krisztina, et al. "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." Immunology Letters (2017).Read more>>
  • Yang J et al. Programmed cell death protein 1 promotes hepatitis B virus transmission through the regulation of ERK1/2-mediated trophoblasts differentiation. Arch Gynecol Obstet. 2019 Dec 2. Read more>>
  • Fei Li. et al. POU2F1 induces the immune escape in lung cancer by up-regulating PD-L1. Am J Transl Res. 2021; 13(2): 672C683Read more>>
  • Mirjana Nacka-Aleksi?. et al. Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment. Exp Gerontol. 2020 Dec;142:11114Read more>>
  • Aussel, Clotilde. et al. IL-1 primed mesenchymal stromal cells moderate hemorrhagic shock-induced organ injuries. Stem Cell Res Ther. 2021 Dec;12(1):1-16Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 30kD. Observed band size:30kD.\n


Lane 1: Hela Cell lysates; Lane 2: Molt-4 Cell Lysates; Lane 3: Jurkat Cell Lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-1867R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Mouse spleen cells were fixed with 70% ice-cold methanol overnight at 4℃ and then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions. The cells were then probed with rabbit anti-PD-1 Polyclonal Antibody, with PE-CY7 conjugation (bs-1867R-PE-CY7) (green) at 1:100 for 30 minutes at room temperature. The graph compares PD-1 antibody to unstained cells (blue) and isotype control (orange).


Lane 1: Rat Raji cell lysates; Lane 2: Mouse Thymus lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Spleen lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Rat Thymus lysates probed with PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.