bs-1867R
[Primary Antibody]
PD-1/CD279 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PD-1/CD279
Immunogen Range: 201-288/288
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5133
Swiss Prot: Q15116
Source: KLH conjugated synthetic peptide derived from human PD-1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 32
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhang, Song-An, et al. "Effect of EBI3 on radiation-induced immunosuppression of cervical cancer HeLa cells by regulating Treg cells through PD-1/PD-L1 pathway." Tumor Biology 39.3 (2017): 1010428317692237.Read more>>
- Szabó, Krisztina, et al. "Expansion of circulating follicular T helper cells associates with disease severity in childhood atopic dermatitis." Immunology Letters (2017).Read more>>
- Yang J et al. Programmed cell death protein 1 promotes hepatitis B virus transmission through the regulation of ERK1/2-mediated trophoblasts differentiation. Arch Gynecol Obstet. 2019 Dec 2. Read more>>
- Fei Li. et al. POU2F1 induces the immune escape in lung cancer by up-regulating PD-L1. Am J Transl Res. 2021; 13(2): 672C683Read more>>
- Mirjana Nacka-Aleksi?. et al. Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment. Exp Gerontol. 2020 Dec;142:11114Read more>>
- Aussel, Clotilde. et al. IL-1 primed mesenchymal stromal cells moderate hemorrhagic shock-induced organ injuries. Stem Cell Res Ther. 2021 Dec;12(1):1-16Read more>>
- Ling Ding. et al. Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity. Cancers. 2021 Jan;13(22):5729Read more>>
- Asuman Feda Bayrak. et al. Association of immune response with overall and disease-free survival in laryngeal squamous cell carcinomas. AM J OTOLARYNG. 2022 Jul;43:103477Read more>>
- Wang, Shan. et al. Excessive Immune Activation and the Correlation with Decreased Expression of PD-1 at the MaternalFetal Interface in Preeclampsia. REPROD SCI. 2022 Jun;:1-11Read more>>
- Xin YAN. et al. PD-1/PD-L1 inhibition promotes hepatic regeneration in small-for-size liver following extended hepatectomy. CYTOKINE. 2022 Nov;159:156017Read more>>
- Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11Read more>>
- Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11Read more>>
- He, Xiaojing. et al. Inhibition of PD-1 Alters the SHP1/2-PI3K/Akt Axis to Decrease M1 Polarization of Alveolar Macrophages in Lung IschemiaCReperfusion Injury. INFLAMMATION. 2022 Nov;:1-16Read more>>
- Li Wang. et al. Radiotherapy upregulated immune checkpoints contribute to the development of second primary OSCC. ORAL DIS. 2023 MayRead more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse colon labeled with Anti-PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 human kidney lysates L2 rat brain lysates probed with Anti PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 30kD. Observed band size:30kD.\n
Lane 1: Hela Cell lysates; Lane 2: Molt-4 Cell Lysates; Lane 3: Jurkat Cell Lysates; Probed with PD-1 Polyclonal Antibody, unconjugated (bs-1867R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse spleen cells were fixed with 70% ice-cold methanol overnight at 4℃ and then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions. The cells were then probed with rabbit anti-PD-1 Polyclonal Antibody, with PE-CY7 conjugation (bs-1867R-PE-CY7) (green) at 1:100 for 30 minutes at room temperature. The graph compares PD-1 antibody to unstained cells (blue) and isotype control (orange).
Lane 1: Rat Raji cell lysates; Lane 2: Mouse Thymus lysates; Lane 3: Mouse Lymph node lysates; Lane 4: Mouse Spleen lysates; Lane 5: Mouse RAW264.7 cell lysates; Lane 6: Rat Thymus lysates probed with PD-1/CD279 Polyclonal Antibody, Unconjugated (bs-1867R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PD-1 ) Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PD-1 ) Polyclonal Antibody, Unconjugated (bs-1867R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.