bs-18726R [Primary Antibody]
phospho-MCL1 (Ser159) Polyclonal Antibody
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Host: Rabbit

Target Protein: phospho-MCL1 (Ser159)

Modification Site: Ser159

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4170

Swiss Prot: Q07820

Source: KLH conjugated synthesised phosphopeptide derived from human Mcl 1 around the phosphorylation site of Ser159.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.

Size: 100uL

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
ICC/IF(1:50-100)

Predicted Molecular Weight: 37 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MCL1 (Ser159) Polyclonal Antibody, Unconjugated (bs-18726R) at 1:200 overnight at 4°C, DAB staining.