bs-1874R [Primary Antibody]
PRDX3/peroxiredoxin 3 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PRDX3/peroxiredoxin 3

Immunogen Range: 161-256/256


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10935

Swiss Prot: P30048

Source: KLH conjugated synthetic peptide derived from human PRDX3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 21


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Hong, et al. "Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprungs disease." International Journal of Experimental Pathology (2014).Read more>>
  • Gaolong Zhong. et al. Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers. J HAZARD MATER. 2023 Sep;458:131908Read more>>
  • Shan Zhang. et al. Synergistic lethality between auranofin-induced oxidative DNA damage and ATR inhibition in cancer cells. LIFE SCI. 2023 Nov;332:122131Read more>>
VALIDATION IMAGES

Lane 1: rat heart lysates Lane 2: rat brain lysates probed with Anti PRDX3/peroxiredoxin 3Polyclonal Antibody, Unconjugated (bs-1874R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 28kD. Observed band size: 28kD.\n


Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Lung tissue lysates; Lane 4: Rat Liver tissue lysates; Lane 5: Rat Lung tissue lysates; Lane 6: Rat Stomach tissue lysates; Lane 7: Human 293T cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human HepG2 cell lysates; Lane 10: Human HeLa cell lysates; Lane 11: Human A549 cell lysates probed with Peroxiredoxin 3 Polyclonal Antibody, Unconjugated (bs-1874R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.