bs-1875R [Primary Antibody]
Profilin 2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Profilin 2

Specificity: This antibody may detect Profilin 1 due to a 71% amino acid sequence similarity in the immunogen sequence.

Immunogen Range: 51-140/140


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 81531

Swiss Prot: Q9EPC6

Source: KLH conjugated synthetic peptide derived from rat Profilin 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human K562 cell lysates probed with Anti-Profilin 2 Polyclonal Antibody (bs-1875R) 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.