bs-1898R [Primary Antibody]
SPATA12 Polyclonal Antibody
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Host: Rabbit

Target Protein: SPATA12

Immunogen Range: 1-100/190


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 353324

Swiss Prot: Q7Z6I5

Source: KLH conjugated synthetic peptide derived from human SPATA12

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

SPATA12, also known as SRG5 (Spermatogenesis-related protein 5), is a 190 amino acid protein that may be involved in the regulation of spermatogenesis. SPATA12 is expressed in normal adult testis, but is absent in fetal testis, implying that it may be involved in the development of testicular maturation. Specifically, SPATA12 is expressed in spermatocytes, spermatids and spermatozoa, with no expression in Leydig and Sertoli cells. Studies with HeLa and GC-1 germ cells indicate that expression of the SPATA12 gene may delay the progression of G1 to S in the cell cycle, therefore it is thought that SPATA12 maintains the cell in a differentiated state and suppresses cell proliferation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 20


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SPATA12 Polyclonal Antibody, Unconjugated (bs-1898R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining DAB staining.