KLH conjugated synthetic peptide derived from human Synphilin 1
Purified by Protein A.
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Store at -20°C for 12 months..
Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
PRODUCT SPECIFIC PUBLICATIONS
- Guo Q et al. Small interfering RNA delivery to the neurons near the amyloid plaques for improved treatment of Alzheimer's disease. Acta Pharm Sin B. 2019 May;9(3):590-603.Read more>>
Rat brain lysates probed with Anti Synphilin-1 Polyclonal Antibody, Unconjugated (bs-1905R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 100kD. Observed band size:60kD 100kD.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Synphilin-1 Polyclonal Antibody, Unconjugated (bs-1905R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synphilin 1 Polyclonal Antibody, Unconjugated (bs-1905R) at 1:200 overnight at 4°C, DAB staining.