bs-19136R [Primary Antibody]
ZFPL1 Polyclonal Antibody
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Host: Rabbit

Target Protein: ZFPL1

Immunogen Range: 31-130/310


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7542

Swiss Prot: O95159

Source: KLH conjugated synthetic peptide derived from human ZFPL1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Zinc finger protein-like 1 is a 310 amino acid single-pass membrane protein with two zinc fingers at the N-terminus, the second of which is likely a RING domain. The RING domain, which is a 40-60 amino acid, cysteine-rich domain that binds two atoms of zinc, plays a key role in the ubiquitination pathway. The presence of zinc finger-like and leucine zipper-like domains in ZFPL1 suggests a role in DNA binding and transcriptional regulation. ZFP1 is widely expressed in the golgi apparatus and is involved in maintaining golgi structure and regulating the rate of cargo transport.

Size: 100µL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ZFPL1 Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ZFPL1 Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ZFPL1 Polyclonal Antibody, Unconjugated (bs-19136R) at 1:400 overnight at 4°C, DAB staining.