bs-19199R [Primary Antibody]
Nephrin (Tyr1217) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nephrin

Specificity: This phosphorylation site is homologous to that of Tyr1232 in Mouse and Tyr1228 Rat.

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4868

Swiss Prot: O60500

Source: KLH conjugated synthetic phosphopeptide derived from human Nephrin around the phosphorylation site of Tyr1217.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)

Predicted Molecular Weight: 136


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney labeled with Anti-Nephrin (Tyr1217) Polyclonal Antibody, Unconjugated (bs-19199R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.