Host: Rabbit
Target Protein: NeuroD1 (Ser274)
Modification Site: Ser274
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4760
Swiss Prot: Q13562
Source: KLH conjugated synthesized phosphopeptide derived from human NeuroD1 around the phosphorylation site of Serine 274
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 40
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Sheep
Pig
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NeuroD1 (Ser274) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NeuroD1 (Ser274) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin-embedded Human Brain Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NeuroD1 (Ser274) Polyclonal Antibody, Unconjugated (bs-19218R) at 1:400 overnight at 4°C, followed by secondary antibody, DAB and counterstaining.
Lane 1: Rat Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with phospho-NeuroD1 Polyclonal Antibody, Unconjugated (bs-19218R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.