bs-1931R [Primary Antibody]
TRPV1/VR1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TRPV1/VR1

Immunogen Range: 800-839/839


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7442

Swiss Prot: Q8NER1

Source: KLH conjugated synthetic peptide derived from human VR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis (By similarity). Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 95


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Assas, Bakri M., et al. "TRPV1 expression and function in splenic dendritic cells: a potential role in immune homeostasis." Immunology (2016).Read more>>
  • Sakakibara A, Sakakibara S, Kusumoto J, Takeda D, Hasegawa T, Akashi M, et al. (2017) Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. PLoS ONE 12(1): e0169723.Read more>>
  • Xu, Shichen, et al. "Capsaicin inhibits metastasis of human papillary thyroid carcinoma BCPAP cells through modulation of the TRPV1 channel." Food & Function (2017).Read more>>
  • Luo, Chengxin, et al. "Upregulation of the transient receptor potential vanilloid 1 in colonic epithelium of patients with active inflammatory bowel disease." Int J Clin Exp Pathol 10.11 (2017): 11335-11344.Read more>>
  • Gökhan ARTAŞ and Tuncay KULOGLU. “Effects of Losartan on TRPV1 and TRPM2 Channels in Experimental Diabetic Rat Kidney Tissue.” Fırat Medical Journal (2014); 19(3): 116-121.Read more>>
  • Gruel, A., el al. “Effects of vitamin D on kidney histology and trpv1 channels in doxorubicin-induced nephropathy.” International Journal of Clinical and Experimental Medicine (2015); 8(8): 13548-55.Read more>>
  • Ghoneum, M.H., et al. “Inhibition of TRPV1 Channel Activity in Human CD4⁺ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576.” Nanomaterials (2018); 8(10): E770.Read more>>
  • Ghoneum et al. Inhibition of TRPV1 Channel Activity in Human CD4⁺ T Cells by Nanodiamond and Nanoplatinum Liquid, DPV576. (2018) Nanomaterials.(Basel). 8 Read more>>
  • Elena Lucarini. et al. Role of Enteric Glia as Bridging Element between Gut Inflammation and Visceral Pain Consolidation during Acute Colitis in Rats. Biomedicines. 2021 Nov;9(11):1671Read more>>
  • Xu Shichen. et al. Capsaicin restores sodium iodine symporter-mediated radioiodine uptake through bypassing canonical TSH?TSHR pathway in anaplastic thyroid carcinoma cells. J Mol Cell Biol. 2021 NovRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat tongue muscle labeled with Anti-TRPV1/VR1 Polyclonal Antibody, Unconjugated (bs-1931R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining


Formalin-fixed and paraffin embedded human endometrial cancer labeled with Anti-TRPV1 Polyclonal Antibody, Unconjugated (bs-1931R) at 1:200 followed by conjugation to the secondary antibody and DAB staining