Mouse ovary lysates probed with WNT5A Polyclonal Antibody, unconjugated (bs-1948R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Mouse uterus lysates probed with WNT5A Polyclonal Antibody, unconjugated (bs-1948R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.


Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-WNT5A Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.


Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Frozen mouse brain sections labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:1000 followed by conjugation to the secondary antibody


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Embryo lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Uterus lysates; Lane 4: Rat Uterus lysates; Lane 5: Rat Ovary lysates; Lane 6: Rat Vas deferens lysates probed with WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with WNT5A Polyclonal Antibody, Unconjugated (bs-1948R) at 1:200 overnight at 4°C, DAB staining.


HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with WNT5A Polyclonal Antibody(bs-1948R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).