bs-1954R [Primary Antibody]
ABCB11 Polyclonal Antibody
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Host: Rabbit

Target Protein: ABCB11

Immunogen Range: 21-120/1321


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 27413

Swiss Prot: Q9QY30

Source: KLH conjugated synthetic peptide derived from mouse BSEP

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.

Size: 100µl

Concentration: Lot Dependent

Applications: ELISA
IHC-F
IF
IHC-P

Predicted Molecular Weight: 146


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shu-Yan Gao. et al. iTRAQ Quantitative Proteomic Analysis of Different Expressed Proteins and Signal Pathways in Bakuchiol-Induced Hepatotoxicity. EVID-BASED COMPL ALT. 2022 Sep 19;2022:292824Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat pancreatic tissue labeled with Anti-BSEP/ABCB11 Polyclonal Antibody, Unconjugated (bs-1954R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BSEP ) Polyclonal Antibody, Unconjugated (bs-1954R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.