bs-19653R [Primary Antibody]
SERCA3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SERCA3

Immunogen Range: 101-200/1043


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 489

Swiss Prot: Q93084

Source: KLH conjugated synthetic peptide derived from human SERCA3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 114


Cross Reactive Species: Human
Xenopus laevis
Xenopus tropicalis

Predicted Cross Reactive Species: Mouse
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane1: jurkat; Lane 2: raji; Lane 3: K562 lysates probed with SERCA3 Polyclonal Antibody, Unconjugated (bs-19653R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human HepG2 cell lysates; Lane 2: Human Raji cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human HL-60 cell lysates probed with SERCA3 Polyclonal Antibody, Unconjugated (bs-19653R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.