bs-19882R-BF488 [Conjugated Primary Antibody]
Parkin (Ser65) Polyclonal Antibody, AbBy Fluor® 488 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Parkin (Ser65)

Modification Site: Ser65

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5071

Swiss Prot: O60260

Source: KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform of SNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in role in regulation of neuron death. Limits the production of reactive oxygen species (ROS). Loss of this ubiquitin ligase activity appears to be the mechanism underlying pathogenesis of PARK2. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. Regulates cyclin-E during neuronal apoptosis. May represent a tumor suppressor gene.

Conjugation: AbBy Fluor® 488

Excitation/ Emission: 499nm/519nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jialong Chen. et al. Rotenone-Induced Neurodegeneration Is Enabled by a p38-Parkin-ROS Signaling Feedback Loop. J Agr Food Chem. 2021;69(46):13942C13952Read more>>
  • Yang, Jie. et al. Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice. Commun Biol. 2022 Feb;5(1):1-17Read more>>
  • Pan Fan. et al. Parkin-mediated mitophagy protects against TNF-_-induced stress in bone marrow mesenchymal stem cells. EXP GERONTOL. 2022 Jul;164:111829Read more>>
  • Dianwen Xu. et al. Staphylococcus aureus induces mitophagy to promote its survival within bovine mammary epithelial cells. VET MICROBIOL. 2023 May;280:109697Read more>>
VALIDATION IMAGES