Host: Rabbit
Target Protein: SMAD5 (Ser463 + Ser465)
Modification Site: Ser463 + Ser465
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4090
Swiss Prot: Q99717
Source: KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 52
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Yang et al. miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. (2018) Int.J.Mol.Med. 42:3115-3124Read more>>
- Yang et al. miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2. (2018) Int.J.Mol.Med. 42:3115-3124Read more>>
- Hui Luo. et al. miR\130a promotes immature porcine Sertoli cell growth by activating SMAD5 through the TGF\\PI3K/AKT signaling pathway. Faseb J. 2020 Nov;34(11):15164-15179Read more>>
- aixiang Wang. et al. Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro. J Zhejiang Univ-Sc B. 2021 May;22(5):410-42Read more>>
Mouse heart lysates probed with SMAD5 (Ser463 + Ser465) Polyclonal Antibody, Unconjugated (bs-19918R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.