bs-20015R [Primary Antibody]
Placental Alkaline Phosphatase Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Placental Alkaline Phosphatase

Immunogen Range: 41-140/535


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 250

Swiss Prot: P05187

Source: KLH conjugated synthetic peptide derived from human Placental alkaline phosphatase 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela cell lysates probed with Placental alkaline phosphatase Polyclonal Antibody, Unconjugated (bs-20015R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Human Hep G2 Cells fixed with 70% methanol (Overnight at 4℃). Cells were then probed with Placental Alkaline Phosphatase Polyclonal Antibody, Unconjugated (bs-20015R) (green) at 1:100 for 30 minutes followed by a PE-conjugated secondary antibody compared to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).