DATASHEET
Host:
Rabbit
Target Protein:
CSFV E2
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
920146
Swiss Prot:
P19712
Source:
CSFV E2 protein
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane.
P7 forms a leader sequence to properly orient NS2 in the membrane.
Uncleaved NS2-3 is required for production of infectious virus.
NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.
NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.
NS4A is a cofactor for the NS3 protease activity.
RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.
VALIDATION IMAGES
Lane 1: Recombinant CSFV E2 protein probed with CSFV E2 Polyclonal Antibody, Unconjugated (bs-20116R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant CSFV E2 protein probed with CSFV E2 Polyclonal Antibody, Unconjugated (bs-20116R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.