bs-20151R [Primary Antibody]
SOCS7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SOCS7

Immunogen Range: 171-270/581


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 30837

Swiss Prot: O14512

Source: KLH conjugated synthetic peptide derived from human SOCS7.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 63


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Du, Yanhua. et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J EXP CLIN CANC RES. 2022 Dec;41(1):1-16Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-SOCS7 Polyclonal Antibody Unconjugated (bs-20151R) at 1:500 followed by conjugation to the secondary antibody and DAB staining


Mouse testis lysates probed with Anti-SOCS7 Polyclonal Antibody, Unconjugated (bs-20151R) at 1:500 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:10000 for 90 min at 37˚C.


Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Mouse Testis lysates; Lane 4: Mouse Stomach lysates; Lane 5: Mouse Placenta lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Cerebellum lysates; Lane 8: Rat Testis lysates; Lane 9: Human HepG2 cell lysates; Lane 10: Human Hela cell lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human HL-60 cell lysates; Lane 13: Human MOLT-4 cell lysates probed with SOCS7 Polyclonal Antibody, Unconjugated (bs-20151R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.