Host: Rabbit
Target Protein: NFKB p65
Immunogen Range: 201-300/551
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5970
Swiss Prot: Q04206
Source: KLH conjugated synthetic peptide derived from human NFKB p65
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
ICC(1:100-500)
Predicted Molecular Weight: 61 kDa
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Wang,et al.Cx43 and NMDA receptors changes in UL122 genetically modified mice hippocampus: a mechanism for spatial memory impairment.(2018) International Journal of Clinical and Experimental Pathology. 11:129-1379.Read more>>
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Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Raji cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.