bs-20226R [Primary Antibody]
FGFR1
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Host: Rabbit

Target Protein: FGFR1

Immunogen Range: 31-130/822


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2260

Swiss Prot: P11362

Source: KLH conjugated synthetic peptide derived from human FGFR1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL (By similarity). Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)

Predicted Molecular Weight: 88


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FGFR1) Polyclonal Antibody, Unconjugated (bs-20226R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Human A673 cell Lysates; Lane 2: Human MOLT4 cell Lysates; Lane 3: Human A549 cell Lysates. Probed with FGFR1 polyclonal Antibody, unconjugated (bs-20226R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.