bs-20322R [Primary Antibody]
CD31 Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD31

Immunogen Range: 251-350/727


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18613

Swiss Prot: Q08481

Source: KLH conjugated synthetic peptide derived from mouse CD31

Purification: purified by Protein A.

Storage Buffer: Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that in humans is encoded by the PECAM1 gene found on chromosome 17. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation.

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-P(1:200-500)
IHC-F(1:200-500)
IF(1:200-500)

Predicted Molecular Weight: 78 kDa


Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cao et al. Danshensu, a major water-soluble component ofSalvia miltiorrhiza, enhances the radioresponse for Lewis Lung Carcinoma xenografts in mice. (2017) Oncol.Lett. 13:605-612Read more>>
  • Zhihao Wang. et al. Rapid Preparation Method for Preparing Tracheal Decellularized Scaffolds: Vacuum Assistance and Optimization of DNase I. Acs Omega. 2021;6(16):10637C10644Read more>>
  • Dai Yarong. et al. METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair. Stem Cells Int. 2021;2021:5512153Read more>>
  • Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359Read more>>
  • Zhou, Liuhua. et al. Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats. Sci Rep-Uk. 2022 Mar;12(1):1-1Read more>>
  • Zhang, Tao. et al. Identification of the key exosomal lncRNAs/mRNAs in the serum during distraction osteogenesis. J ORTHOP SURG RES. 2022 Dec;17(1):1-13Read more>>
  • Kyu Young Choi. et al. A digital light processing 3D-printed artificial skin model and full-thickness wound models using silk fibroin bioink. ACTA BIOMATER. 2023 ApRead more>>
  • Cristiane M. Franca. et al. High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration. ADV HEALTHC MATER. 2023 May;:220284Read more>>
  • Lubin Zhou. et al. Electrospun Self-Pumping dressing with gastrodin for immunomodulation and rapid healing of diabetic wounds. CHEM ENG J. 2024 Sep;495:153424Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD31 Polyclonal Antibody, Unconjugated(bs-20322R) at 1:500 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.