bs-2032R [Primary Antibody]
PMP2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PMP2

Immunogen Range: 31-132/132


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5375

Swiss Prot: P02689

Source: KLH conjugated synthetic peptide derived from human PMP2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

PMP2 is a small basic protein, belonging to the fatty-acid binding protein (FABP) family and is one of the major proteins of peripheral myelin. It appears to be involved in the transport of fatty acids or the metabolism of myelin lipids, and has been shown to have lipid-binding activity. PMP2 protein may have an important role in the organization of compact myelin.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 15


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-PMP2 Polyclonal Antibody, Unconjugated (bs-2032R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin-embedded rat brain labeled with Anti-PMP2 Polyclonal Antibody, Unconjugated(bs-2032R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.


SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PMP2) polyclonal Antibody, Unconjugated (bs-2032R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.