bs-2033R [Primary Antibody]
E.coli DH-5 Alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E.coli DH-5 Alpha

Clonality: Polyclonal

Isotype: IgG

Source: Whole cell protein of Ecoli DH-5 Alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: This antibody reacts with Escherichia coli HD5a (E.coli HD5a), Escherichia coli is a gram-negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins. E. coli DH5_ strain is an engineering strain widely used in biopharmaceuticals. According to the requirements of product quality inspection, it is necessary to detect the residual amount of DH5_ bacterial protein in biological products. This product is an anti-serum obtained by immunizing rabbits with DH5_ bacterial protein as an antigen, and purified by affinity chromatography to obtain antibody IgG, which can be used for ELISA, Western Blot and other experimental tests.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Bacteria
Others
(Escherichia coli)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li, Yiwei, et al. "Agarose-based microfluidic device for rapid point-of-care concentration and detection of pathogen." Analytical Chemistry (2014).Read more>>
VALIDATION IMAGES

Antigen: bs-2033P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in MicroplateReader by 450nm.


Lane 1: E.coli lysates; Lane 2: E.coli lysates; Probed with E.coli DH-5 Alpha Polyclonal Antibody, Unconjugated (bs-2033R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 60 min at 37˚C.