bs-20355R
[Primary Antibody]
NFKB p65 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: NFKB p65
Immunogen Range: 201-300/551
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5970
Swiss Prot: Q04206
Source: KLH conjugated synthetic peptide derived from human NFKB p65
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF()
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wu Z et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019 Nov 15;10:2615.Read more>>
- Xiaoyan Hu. et al. Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. Mol Med Rep. 2020 Sep;22(3):2003-2011Read more>>
- Yongli Zhang. et al. Licorice extract ameliorates hyperglycemia through reshaping gut microbiota structure and inhibiting TLR4/NF-_B signaling pathway in type 2 diabetic mice. Food Res Int. 2022 Mar;153:110945Read more>>
- Wenyang Wang. et al. Glycogen metabolism reprogramming promotes inflammation in coal dust-exposed lung. ECOTOX ENVIRON SAFE. 2022 Sep;242:113913Read more>>
- Jiaojiao Wu. et al. The involvement of Pellino-1 downregulation in the modulation of visceral hypersensitivity via the TLR4/NF-_B pathway in the rat fastigial nucleus. NEUROSCI LETT. 2022 Sep;787:136815Read more>>
- Guirong Chen. et al. The interaction of MD-2 with small molecules in huanglian jiedu decoction play a critical role in the treatment of sepsis.. FRONT PHARMACOL. 2022 Sep;13:947095-947095Read more>>
- Xuezhi Zhang. et al. Natural emodin reduces myocardial ischemia/reperfusion injury by modulating the RUNX1/miR?142?3p/DRD2 pathway and attenuating inflammation. EXP THER MED. 2022 Dec;24(6):1-11Read more>>
- Chen Zhang. et al. The novel hyaluronic acid granular hydrogel attenuates osteoarthritis progression by inhibiting the TLR-2/NF-B signaling pathway through suppressing cellular senescence. BIOENG TRANSL MED. 2022 Dec;:e10475Read more>>
- Yanjie Wang. et al. Inhibitory Effect of Bisdemethoxycurcumin on DNCB-Induced Atopic Dermatitis in Mice. MOLECULES. 2023 Jan;28(1):293Read more>>
- Gao Jie-Fang. et al. Myricetin treatment has ameliorative effects in DNFB-induced atopic dermatitis mice under high-fat conditions. TOXICOL SCI. 2022 DecRead more>>
- Yang Liu. et al. Isochlorogenic Acid C Restrains Erk/JNK/NF-B Signaling to Alleviate Inflammatory Response and Promote Cell Apoptosis. J FOOD BIOCHEM. 2023;2023:5547108Read more>>
- Fengjuan Chen. et al. JNK molecule is a toxic target for IPEC-J2 cell barrier damage induced by T-2 toxin. ECOTOX ENVIRON SAFE. 2023 Sep;263:115247Read more>>
- Jing Zhang. et al. Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-B and restoring lipid production in Caco-2 cell line. J FUNCT FOODS. 2023 Aug;107:105709Read more>>
- Xing Zeyu. et al. KPNA2 Silencing, Regulated by E3 Ubiquitin Ligase FBXW7, Alleviates Endothelial Dysfunction and Inflammation Through Inhibiting the Nuclear Translocation of p65 and IRF3: A Possible Therapeutic Approach for Atherosclerosis. INFLAMMATION. 2023 Jul;:1-18Read more>>
- Guoyong Zhang. et al. Buyang Huanwu Decoction suppresses cardiac inflammation and fibrosis in mice after myocardial infarction through inhibition of the TLR4 signalling pathway. J ETHNOPHARMACOL. 2023 Nov;:117388Read more>>
- Yuhan Lin. et al. Puerarin inhibits cisplatin-induced ototoxicity in mice through regulation of TRPV1-dependent calcium overload. BIOCHEM PHARMACOL. 2024 Feb;220:115962Read more>>
VALIDATION IMAGES
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NFKB p65 Polyclonal Antibody(bs-20355R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat lymph node; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20355R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20355R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20355R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NFKB p65) polyclonal Antibody, Unconjugated (bs-20355R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (NFKB p65) polyclonal Antibody, Unconjugated (bs-20355R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.