bs-20373R [Primary Antibody]
IL-10 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-10

Immunogen Range: 51-100/178


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3586

Swiss Prot: P22301

Source: KLH conjugated synthetic peptide derived from human IL-10

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. IL-10 Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 20


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li C et al. Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients. Int Immunopharmacol. 2019 Aug;73:539-551. Read more>>
  • Teng Ma. et al. Interleukin?18 and ?10 may be associated with lymph node metastasis in breast cancer. Oncol Lett. 2021 Apr;21(4):1-1Read more>>
  • Guangcong Peng. et al. Intranasal administration of DHED protects against exhaustive exercise-induced brain injury in rats. Brain Res. 2021 Dec;1772:147665Read more>>
  • Liu Z. et al. A Novel Hydrogen Sulfide Donor Reduces Pilocarpine-Induced Status Epilepticus and Regulates Microglial Inflammatory Profile.. Front Cell Neurosci. 2021 Dec;15:780447-780447Read more>>
  • Qiyun Kang. et al. A novel hydrogen sulfide donor reduces neuroinflammation and seizures by activating ATP-sensitive potassium channels. NEUROSCI RES. 2023 JulRead more>>
  • Kar Ezgi. et al. Prophylactic and Therapeutic Efficacy of Boric Acid on Lipopolysaccharide-Induced Liver and Kidney Inflammation in Rats. BIOL TRACE ELEM RES. 2023 Nov;:1-13Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin-embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes, DAB and counterstaining.


Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Lane 1: Rat Blood Plasma; Lane 2: Rat Blood Plasma; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Mouse Raw264.7 Cells; Probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Lane 1: Recombinant human IL-10 protein, His (HEK293)(bs-43053P) probed with IL-10 Polyclonal Antibody, Unconjugated (bs-20373R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.