bs-2040R [Primary Antibody]
Atrial natriuretic factor Polyclonal Antibody
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Host: Rabbit

Target Protein: Atrial natriuretic factor

Immunogen Range: 124-151/153


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4878

Swiss Prot: P01160

Source: KLH conjugated synthetic peptide derived from human ANP:124-151/153

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Straub, Rainer H., et al. "Increased extracellular water measured by bioimpedance and by increased serum levels of atrial natriuretic peptide in RA patients—signs of volume overload." Clinical Rheumatology (2016): 1-11Read more>>
  • Malinowski, Nadezhda. Erhöhtes extrazelluläres Wasser gemessen durch Bioimpedanz und erhöhter Serumspiegel von atrialem natriuretischen Peptid bei rheumatoider Arthritis–Anzeichen von Volumenüberlastung. Diss. 2015.Read more>>
  • Ruomiao Li. et al. Protective effects of dioscin against isoproterenol-induced cardiac hypertrophy via adjusting PKC/ERK-mediated oxidative stress. Eur J Pharmacol. 2021 Sep;907:174277Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-ANP Polyclonal Antibody, Unconjugated (bs-2040R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Lane 1: Heart (Mouse); Lane 2: Heart (Rat) lysates probed with ANP Polyclonal Antibody, Unconjugated (bs-2040R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Atrial natriuretic factor Polyclonal Antibody, Unconjugated (bs-2040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Atrial natriuretic factor Polyclonal Antibody, Unconjugated (bs-2040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Atrial natriuretic factor Polyclonal Antibody, Unconjugated (bs-2040R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.