bs-2043R [Primary Antibody]
CK18 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CK18

Immunogen Range: 53-150/430


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3875

Swiss Prot: P05783

Source: KLH conjugated synthetic peptide derived from human CK18

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Pengfei, et al. "Administration of BMSCs with Muscone in Rats with Gentamicin-Induced AKI Improves Their Therapeutic Efficacy." PLoS One 9.5 (2014): e97123.Read more>>
  • Liu et al. Therapeutic effects of suppressors of cytokine signaling in diabetic nephropathy. (2014) J.Histochem.Cytoche. 62:119-28Read more>>
  • Fagundes et al. Binge Drinking of Ethanol during Adolescence Induces Oxidative Damage and Morphological Changes in Salivary Glands of Female Rats. (2016) Oxid.Med.Cell.Longe. 2016:7323627Read more>>
  • Li et al. Hyperglycaemia exacerbates choroidal neovascularisation in mice via the oxidative stress-induced activation of STAT3 signalling in RPE cells. (2012) PLoS.One. 7:e47600Read more>>
  • Gao R et al. Protective effect of chlorogenic acid on lipopolysaccharide-induced inflammatory response in dairy mammary epithelial cells.Microb Pathog. 2018 Jul 24;124:178-182.Read more>>
  • Yu Zhong. et al. LIGHT aggravates sepsis\associated acute kidney injury via TLR4\MyD88\NF\B pathway. J Cell Mol Med. 2020 Oct;24(20):11936-11948Read more>>
  • Fei Sun. et al. Directly construct microvascularization of tissue engineering trachea in orthotopic transplantation. Mat Sci Eng C-Mater. 2021 May;:112201Read more>>
  • Qihui Chu. et al. High-Specific Isolation and Instant Observation of Circulating Tumour Cell from HCC Patients via Glypican-3 Immunomagnetic Fluorescent Nanodevice. Int J Nanomed. 2021 Jun;16:4161-4173Read more>>
  • Zhong Heju. et al. Methionine Protects Mammary Cells against Oxidative Stress through Producing S-Adenosylmethionine to Maintain mTORC1 Signaling Activity. Oxid Med Cell Longev. 2021;2021:5550196Read more>>
  • Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
  • Jinghan Yang. et al. Regularity of Toll-Like Receptors in Bovine Mammary Epithelial Cells Induced by Mycoplasma bovis. FRONT VET SCI. 2022; 9: 8467Read more>>
  • Xu Bai. et al. Toll-like Receptor 2 Is Associated with the Immune Response, Apoptosis, and Angiogenesis in the Mammary Glands of Dairy Cows with Clinical Mastitis. INT J MOL SCI. 2022 Jan;23(18):10717Read more>>
  • Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 ApRead more>>
  • Jie Liu. et al. dCas9-guided demethylation of the AKT1 promoter improves milk protein synthesis in a bovine mastitis mammary gland epithelial model induced by using?Staphylococcus aureus. CELL BIOL INT. 2023 DecRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded pig liver labeled with Anti-CK18 Polyclonal Antibody, Cy3 Conjugated (bs-2043R-Cy3) at 1:200 for 60 minutes at 37C.


PC12 cells probed with Rabbit Anti-CK18 Polyclonal Antibody (bs-2043R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.


Paraformaldehyde-fixed, paraffin embedded human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CK18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Rat Large intestine lysates probed with Cytokeratin 18 Polyclonal Antibody, Unconjugated (bs-2043R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Tissue/cell:A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 18) polyclonal Antibody, Unconjugated (bs-2043R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.