bs-20492R [Primary Antibody]
Phospho-IRAK1 (Thr209) Polyclonal Antibody
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Host: Rabbit

Target Protein: Phospho-IRAK1 (Thr209)

Modification Site: Thr209

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3654

Swiss Prot: P51617

Source: KLH conjugated synthesised phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr209

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Size: 100uL

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 78 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-IRAK1 (Thr209)) polyclonal Antibody, Unconjugated (bs-20492R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.