bs-20495R [Primary Antibody]
Tubulin, Alpha 4a Polyclonal Antibody
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Host: Rabbit

Target Protein: Tubulin, Alpha 4a

Immunogen Range: 41-140/448


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7277

Swiss Prot: P68366

Source: KLH conjugated synthetic peptide derived from human Tubulin, Alpha 4a

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 50 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Stomach lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Rat Kidney lysates probed with Tubulin, Alpha 4a Polyclonal Antibody, Unconjugated (bs-20495R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.