bs-20497R [Primary Antibody]
Leptin receptor Polyclonal Antibody
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Host: Rabbit

Target Protein: Leptin receptor

Immunogen Range: 561-660/1165


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3953

Swiss Prot: P48357

Source: KLH conjugated synthetic peptide derived from human Leptin receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Size: 100uL

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 103/132 kDa


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Leptin receptor Polyclonal Antibody(bs-20497R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).