bs-2054R [Primary Antibody]
Nkx2.5 Polyclonal Antibody
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Host: Rabbit

Target Protein: Nkx2.5/Cardiac-specific homeobox 1

Immunogen Range: 85-180/324


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18091

Swiss Prot: P42582

Source: KLH conjugated synthetic peptide derived from mouse Nkx2.5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ruan, Zhong-Bao, et al. "Inhibitor of p53–p21 pathway induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyogenic cells." Cytotechnology (2015): 1-9.Read more>>
  • Kim CW et al. Effects of cigarette smoke components on myocardial differentiation of mouse embryonic stem cells. Environ Toxicol. 2019 Sep 10.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat heart labeled with Anti-Nkx2.5/Cardiac-specific homeobox 1 Polyclonal Antibody, Unconjugated (bs-2054R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nkx2.5 Polyclonal Antibody, Unconjugated (bs-2054R) at 1:200 overnight at 4°C, DAB staining.