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Host: Rabbit
Target Protein: HDAC9
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 9734
Swiss Prot: Q9UKV0
Source: KLH conjugated synthetic peptide derived from human HDAC9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
IF(IF=1:100-500)
Predicted Molecular Weight: 111
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Gaoyan Wang. et al. A novel long noncoding RNA, LOC440173, promotes the progression of esophageal squamous cell carcinoma by modulating the miR\30d\5p/HDAC9 axis and the epithelialCmesenchymal transition. 2020 Oct 2Read more>>
- Derya Erisik. et al. Differences and Similarities between Colorectal Cancer Cells and Colorectal Cancer Stem Cells: Molecular Insights and Implications. ACS OMEGA. 2023;XXXX(XXX):XXX-XXXRead more>>
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HDAC9 Polyclonal Antibody, Unconjugated (bs-20554R) at 1:400 overnight at 4°C, DAB staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with HDAC9 Polyclonal Antibody, Unconjugated (bs-20554R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hepg2 cell lysates probed with HDAC9 Polyclonal Antibody, Unconjugated (bs-20554R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat testis）; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (HDAC9) Polyclonal Antibody, Unconjugated (bs-20554R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.