Host: Rabbit
Target Protein: SOX10
Immunogen Range: 201-300/466
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6663
Swiss Prot: P56693
Source: KLH conjugated synthetic peptide derived from human SOX10
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 50
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Sheep
Pig
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Yun Wang. et al. Prucalopride might improve intestinal motility by promoting the regeneration of the enteric nervous system in diabetic rats. INT J MOL MED. 2022 Jul;50(1):1-1Read more>>
- Gill Viktor T. et al. Multiorgan Involvement of Dormant Uveal Melanoma Micrometastases in Postmortem Tissue From Patients Without Coexisting Macrometastases. AM J CLIN PATHOL. 2023 ApRead more>>
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with SOX10 Polyclonal Antibody, Unconjugated (bs-20563R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum; Probed with SOX10 Polyclonal Antibody, Unconjugated (bs-20563R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Rat Colon tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 minutes; Antibody incubation with SOX10 Polyclonal Antibody, Unconjugated (bs-20563R) at 1:500 overnight at 4°C, followed by a conjugated secondary antibody, DAB and counter staining.
B16 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SOX10 Polyclonal Antibody(bs-20563R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).